Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7610072 | Journal of Chromatography A | 2016 | 12 Pages |
Abstract
Currently, no analytical method is available to demonstrate progesterone administration in biological samples collected in rearing animals, and therefore, tracking the abuse of this popular growth promoter is arduous. In this study, a method is presented to reveal progesterone (PG) treatment on the basis of carbon isotope measurement of 5β-pregnane-3α, 20α-diol (BAA-PD), a major PG metabolite excreted in bovine urine, by gas chromatography-mass spectrometry/combustion/isotope ratio mass spectrometry (GC-MS/C/IRMS). 5-Androstene-3β,17α-diol (AEdiol) is used as endogenous reference compound. Intermediate precisions (n = 11) of 0.56Ⱐand 0.68Ⱐhave been determined for AEdiol and BAA-PD, respectively. The analytical method was used for the very first time to successfully differentiate urine samples collected in treated and untreated animals.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Geert Janssens, Sven Mangelinckx, Dirk Courtheyn, Norbert De Kimpe, Bert Matthijs, Bruno Le Bizec,