Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7610395 | Journal of Chromatography A | 2016 | 8 Pages |
Abstract
A high throughput and low cost approach to separate free fatty acids (FFAs) from phospholipid and acylglycerols (esterified fatty acids, EFAs) has been demonstrated, which may be widely used as a sample preparation method in the metabolomics and lipid research. The optimal conditions for FFAs reacting with N-hydroxysuccinimide (NHS) only need 10Â min at room temperature to obtain a 93.5% yield of FFAs-NHS ester. The rest 6% FFA transformed into N-cyclohexyl-fatty acid-amide which is stable to methyl esterification adopted for fatty acids analysis. 10Â min are taken for FFAs-NHS ester to react with amino functionalized silica nanoparticles to immobilize the FFAs. The separation of FFAs from EFAs could be carried out readily by centrifugation. The whole process including derivatization, immobilization, and centrifugation takes less than 40Â min. Much more accurate fatty acids composition of rat plasma EFAs could be obtained by this approach than the previous reported methods.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jun Chen, Qiang Lyu, Mingqing Yang, Zhi Chen, Junhui He,