Human neutrophil elastase inhibition studied by capillary electrophoresis with laser induced fluorescence detection and microscale thermophoresis

Furthermore, MST technique was used for evaluating HNE interaction with the ursolic acid. Up to 16 capillaries were automatically processed to obtain in one titration experiment the dissociation constant for the HNE-ursolic acid complex. Ki was found to be 2.72 ± 0.66 μM (n = 3) which is in excellent agreement with the value determined by CE enzyme inhibition studies (Ki = 2.81 μM) confirming the reliability of the developed CE assay and the competitive inhibition mode of ursolic acid.