Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7610997 | Journal of Chromatography A | 2015 | 23 Pages |
Abstract
Dextran, a family of natural polysaccharides, consists of an α (1â6) linked-glucose main (backbone) chain having a number of branches. The determination of the types and the quantities of branches in dextran is important in understanding its various biological roles. In this study, a hyphenated method using high-performance anion exchange chromatography (HPAEC) in parallel with pulsed amperometric detection (PAD) and mass spectrometry (MS) was applied to qualitative and quantitative analysis of dextran branches. A rotary cation-exchange cartridge array desalter was used for removal of salt from the HPAEC eluent making it MS compatible. MS and MS/MS were used to provide structural information on the enzymatically prepared dextran oligosaccharides. PAD provides quantitative data on the ratio of enzyme-resistant, branched dextran oligosaccharides. Both the types and degree of branching found in a variety of dextrans could be simultaneously determined online using this method.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Lin Yi, Yilan Ouyang, Xue Sun, Naiyu Xu, Robert J. Linhardt, Zhenqing Zhang,