Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7611080 | Journal of Chromatography A | 2015 | 12 Pages |
Abstract
A novel method for online extraction, pH-gradient separation, and analysis of nine non-steroidal anti-inflammatory drugs (NSAIDs) was developed by coupling online eluent-switching technique to single anion-exchange chromatographic column/ion trap mass spectrometer (MS) and used for monitoring NSAIDs residues in pig serum. A neutral eluent and a pH-gradient eluent were used for extraction and separation of NSAIDs, respectively. Each of nine NSAIDs has an MS precursor ion of either [MâH]â or [MâNa]â. The extracted ion chromatogram for a specific product ion of each NSAID was used for its quantitative analysis. The dynamic linear ranges of calibration curves were all 0-200 ng mLâ1 (R2 > 0.9950). The analysis accuracies estimated by spiking standard concentrations at 20, 100, and 200 ng mLâ1 were 80.5-99.9%. The corresponding intra-day and inter-day precisions (RSD%) were 2.5-14.5% and 2.9-15.2%, respectively. The limit of detection/limit of quantitation of NSAIDs were 1.3/4.3, 0.5/1.6, 0.2/0.5, 2.5/8.2, 1.5/4.9, 0.6/2.1, 0.6/2.0, 0.5/1.7, and 0.6/2.1 ng mLâ1 for carprofen, diclofenac, flunixin, ibuprofen, ketoprofen, meclofenamic acid sodium, mefenamic acid, niflumic acid, and tolfenamic acid, respectively. After 1 h injection of a dose containing 2 mg kgâ1 weight pig of flunixin and tolfenamic acid to the pigs, a residue amount of 3480 ± 36 ng mLâ1 and 431 ± 13 ng mLâ1, respectively, was reached for the incurred pig serum specimens and both residues were reduced to about 20 ng mLâ1 at the time of 24 h.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Kai Chun Chang, Jyh Shiun Lin, Cheanyeh Cheng,