Comparison of the most recent chromatographic approaches applied for fast and high resolution separations: Theory and practice

Finally, to attain the highest possible kinetic performance in SFC, there is a need to further reduce extra-column band broadening (system dispersion, σext2) down to 10 μL2 or less. In addition, the system upper pressure limit should be extended to ∼800 bar, to have a sufficient flexibility, particularly when working with a mobile phases containing a relatively high amount of organic solvent (e.g. 20-40% MeOH).