Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7611571 | Journal of Chromatography A | 2015 | 11 Pages |
Abstract
A powerful and sensitive method, by microextraction packed sorbent (MEPS), and ultra-high performance liquid chromatography (UHPLC) with a photodiode array (PDA) detection, is described for the determination of fluoxetine, clomipramine and their active metabolites in human urine samples. The MEPS variables, such as sample volume, pH, number of extraction cycles (draw-eject), and desorption conditions (solvent and solvent volume of elution) were optimized. The analysis were carried out using small sample volumes (500 μL) and in a short time period (5 min for the entire sample preparation step). Good linearity was obtained for all antidepressants with the correlation coefficients (R2) above 0.9965. The limits of detection (LOD) ranged from 0.068 to 0.087 μg mLâ1. The recoveries were from 93% to 98%, with relative standard deviations less than 6%. The inter-day precision, expressed as the relative standard deviation, varied between 3.8% and 8.5% while the intra-day precision between 3.0% and 7.1%. In order to evaluate the proposed method for clinical use, the MEPS/UHPLC-PDA method was applied to analysis of urine samples from depressed patients.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Vera Alves, João Gonçalves, Carlota Conceição, Helena M. Teixeira, José S. Câmara,