Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7611834 | Journal of Chromatography A | 2015 | 10 Pages |
Abstract
Co-administration of opioids with benzodiazepines is very common around the world. A semi-automated method was developed for the determination of four opioids and two benzodiazepines as well as their metabolites (including glucuronide metabolites) in human urine, based on on-line column-switching-solid-phase extraction (CS-SPE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The CS-SPE was performed by loading 200 μL of urine sample to an Oasis HLB cartridge. Detection was achieved using a LC-MS/MS system equipped with an electrospray ionization source (ESI). For unequivocal identification and confirmation, two selected reaction monitoring transitions were registered for each compound, and no co-elution of interferences was observed at the expected retention time. Significant ion suppressions were observed for most analytes during chromatographic runs, but isotope-labeled internal standards (ISs) were used and found to be useful to compensate for the determination error caused by the matrix effect. The assay's linearity ranged from 1-20 ng/mL to 800-1000 ng/mL for 23 compounds, except for lorazepam (LOR), whose linearity was in the range of 1-100 ng/mL. This method showed to be precise and accurate. The relative standard deviation (RSD) % values of within-run precision, between-run precision and total precision were not greater than 10.4% (n = 3), 12.9% (n = 5) and 15.1% (n = 15), respectively. Accuracy values were in the range of 87.5-110%. Limits of detection (LODs) ranged from 0.2 ng/mL to 5 ng/mL, and limits of quantification (LOQs) ranged from 1 ng/mL to 20 ng/mL. The method was applied to the assay of 12 samples from forensic cases, which exemplified the co-administration of benzodiazepines (BZDs) by some heroin abusers. This method was of high sensitivity, selectivity and reliability, minimum sample manipulation, semi-automation, and fairly high throughput (analysis time per sample was 20 min). The method developed will be useful for the detection of co-administrated drugs and the study of the interactions of BDZs with opioids.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Lingjuan Xiong, Rong Wang, Chen Liang, Xiaomei Teng, Fengli Jiang, Libo Zeng, Haiying Ye, Chunfang Ni, Xiaoliang Yuan, Yulan Rao, Yurong Zhang,