Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7611913 | Journal of Chromatography A | 2015 | 12 Pages |
Abstract
The zirconia-coated silica (ZrO2/SiO2) material was obtained by coupling layer-by-layer (LbL) self-assembly method and sol-gel technology, to take dual advantages of the suitable porous structure of SiO2 and basic resistance of ZrO2. Adenosine 5â²-monophosphate (5â²-AMP) was then self-assembled onto ZrO2/SiO2 via Lewis acid-base interaction, generating 5â²-AMP-ZrO2/SiO2. The chromatographic properties of 5â²-AMP-ZrO2/SiO2 were systemically studied by evaluating the effect of acetonitrile content, pH and buffer concentration in the mobile phase. The results demonstrated that the 5â²-AMP-ZrO2/SiO2 possessed hydrophilic interaction chromatographic (HILIC) property comprising hydrophilic, hydrogen-bonding, electrostatic and ion-exchange interactions. For basic analytes, the column efficiency of ZrO2/SiO2 and 5â²-AMP-ZrO2/SiO2 was superior to the bare ZrO2, and different selectivity was obtained after the introduction of 5â²-AMP. For acidic analytes, good resolution was obtained on 5â²-AMP-ZrO2/SiO2 while the analysis failed on the bare ZrO2 column owing to strong adsorption. Hence, the proposed 5â²-AMP-ZrO2/SiO2 had great potential in analyzing acidic compounds in HILIC mode. It was an extended application of ZrO2 based SP.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Qing Wang, Zhi-Yuan Luo, Mao Ye, Yu-Zhuo Wang, Li Xu, Zhi-guo Shi, Lanying Xu,