Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7612324 | Journal of Chromatography A | 2014 | 4 Pages |
Abstract
At high flow rates and pressures, columns packed with sub-2 μm particles suffer from efficiency losses due to frictional heating. The thermal environment of the column (insulated or isothermal) can decrease or magnify these losses. While a number of studies have been conducted demonstrating the improved performance (partially due to the benefits of enhanced thermal conductivity) of columns packed with superficially porous particles, none have made a comparison between sub-2 μm fully and superficially porous particles in an isothermal environment where radial thermal gradients are maximized and thermal broadening is amplified. Here we show that when such columns are characterized in a recirculating water jacket (providing an isothermal environment), efficiency loss and changes in retention and mobile phase temperature are reduced for sub-2 μm superficially porous particles compared to sub-2 μm fully porous particles.
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Authors
James P. Grinias, Dayley S. Keil, James W. Jorgenson,