Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7612366 | Journal of Chromatography A | 2014 | 7 Pages |
Abstract
Peptide imprinted polymers were developed for detection of progastrin releasing peptide (ProGRP); a low abundant blood based biomarker for small cell lung cancer. The polymers targeted the proteotypic nona-peptide sequence NLLGLIEAK and were used for selective enrichment of the proteotypic peptide prior to LCMS based quantification. Peptide imprinted polymers with the best affinity characteristics were first identified from a 96-polymer combinatorial library. The effects of functional monomers, crosslinker, porogen, and template on adsorption capacity and selectivity for NLLGLIEAK were investigated and optimized. Ultimately, a solid phase extraction method was developed for highly selective enrichment of the target peptide from tryptic digests.
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Physical Sciences and Engineering
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Analytical Chemistry
Authors
Abed Abdel Qader, Javier Urraca, Silje Bøen Torsetnes, Finn Tønnesen, Léon Reubsaet, Börje Sellergren,