Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7612422 | Journal of Chromatography A | 2014 | 6 Pages |
Abstract
pH-zone-refining counter-current chromatography was successfully applied to the preparative separation of five quaternary ammonium alkaloids from the crude extract of Coptis chinensis Franch. The separation was performed with a two-phase solvent system composed of chloroform-methanol-water (4:3:3, v/v), where the upper aqueous stationary phase was added with 60Â mM of hydrochloric acid and the lower organic mobile phase with 5Â mM of triethylamine. From 1.0Â g of crude extract, 5.4Â mg of columbamine at 96.6% purity, 6.1Â mg of jateorhizine at 98.8% purity, 58.3Â mg of coptisine at 99.5% purity, 25.6Â mg of palmatine at 98.4% purity and 503.9Â mg of berberine at 99.5% purity were obtained. The purities of the isolated alkaloids were analyzed by HPLC and the chemical structures were identified by electrospray ionization-mass spectrometry and 1H NMR.
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Authors
Changlei Sun, Jia Li, Xiao Wang, Wenjuan Duan, Tianyou Zhang, Yoichiro Ito,