Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7612479 | Journal of Chromatography A | 2014 | 8 Pages |
Abstract
In this work, we have generalized the use of capillary isotachophoresis as a universal method for determination of effective charge of anionic and cationic (co)polymers on ordinary capillary electrophoresis instruments. This method is applicable to a broad range of strong or weak polyelectrolytes with good repeatability. Experimental parameters (components and concentrations of leading and terminating electrolytes, capillary diameters, constant electric current intensity) were optimized for implementation in 100 μm i.d. capillaries for both polyanions and polycations. Determined values of polymer effective charge were in a very good agreement with those obtained by capillary electrophoresis with indirect UV detection. Uncertainty of the effective charge measurement using isotachophoresis was addressed and estimated to be â¼5-10% for solutes with mobilities in the 20-50 Ã 10â9 m2 Vâ1 sâ1 range.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Joseph Chamieh, DuÅ¡an Koval, Adeline Besson, Václav KaÅ¡iÄka, Hervé Cottet,