Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7612543 | Journal of Chromatography A | 2014 | 9 Pages |
Abstract
A new analytical method was developed and validated for simultaneous analysis of 27 phthalates in milk and milk products. Response surface methodology was employed to optimize a quick, easy, cheap, effective, rugged, and safe (QuEChERS) sample preparation method. Ultrahigh-performance liquid chromatography and electrospray ionization quadrupole Orbitrap high-resolution mass spectrometry (UHPLC/ESI Q-Orbitrap) was used for the separation and detection of all the analytes. The method was validated by taking into consideration the guidelines specified in Commission Decision 2002/657/EC and 2007/19/EC. The extraction recoveries were in a range of 90.7% to 104.6%, with coefficient of variation <5.6%. The 27 compounds behave dynamic range in the 0.1-1000 μg kgâ1 concentration, with correlation coefficient >0.99. The limits of detection for the analytes are in the range 0.32-2.6 μg kgâ1. This method has been successfully applied on screening of phthalates in 96 commercial milk and milk product samples.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Wei Jia, Xiaogang Chu, Yun Ling, Junrong Huang, James Chang,