Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7613318 | Journal of Chromatography A | 2014 | 4 Pages |
Abstract
(R,R)-oxaliplatin is an anticancer enantiopure active pharmaceutical ingredient. Little attention has been devoted to the analysis of its enantiomeric composition. The enantioselective HPLC method reported in the current Pharmacopoeias shows clear disadvantages with regard to the low resolution and long elution times. In this work, it has been proven the applicability of a last generation polysaccharide-based chiral stationary phase (CSP), i.e. the Chiralpak IC-3, in the enantioseparation of oxaliplatin. Experimental results demonstrated the benefits arising from the development of enantioselective hydrophilic interaction liquid chromatography (HILIC) based strategies. A baseline separation with resolution factor of 5.8 was achieved using a 100 mm Ã 4.6 mm I.D. IC-3 column set at the temperature of 40 °C and a mobile phase consisting of acetonitrile-water 100:5 mixture. At a flow rate of 1 mL minâ1 the separation was completed within 8 min. The optimized method was proven to be sensitive with LOD and LOQ of the enantiomeric impurity of 0.07 and 0.21 μg mLâ1, respectively.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Bruno Gallinella, Lucia Bucciarelli, Leo Zanitti, Rosella Ferretti, Roberto Cirilli,