Article ID Journal Published Year Pages File Type
7613863 Journal of Chromatography A 2014 6 Pages PDF
Abstract
The growing scientific attention in the biological function of D-amino acids leads to an increasing analytical interest for enantiomeric amino acid separation, which is still very challenging due to the lack of sufficiently sensitive, high-throughput analytical methods that can cope with often occurring matrix interferences and very low D-amino acid concentrations. Here, enantioseparation can benefit from improved resolution and chromatographic speed offered by modern UHPLC techniques and the precision of MS detection. We developed a RP-UHPLC-QqToF-MS method using pre-column OPA/IBLC derivatization for very precise discrimination of amino acids enantiomers. The method shows a superb sensitivity with limits of detection in the range of several pmol/l. It has neither shown matrix inferences in the tested very complex biological matrices (serum, plasma, urine and gut) nor stability or racemization problems.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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