Influence of amino acid moiety accessibility on the chiral recognition of cyclodextrin-amino acid mixed selectors in enantioselective gas chromatography

► Original mixed selectors were synthesized by coupling amino acid on cyclodextrin. ► Structures were designed to limit interactions between l-valine and cyclodextrin. ► Mixed selectors exhibited better enantioselectivity than Chirasil-l-Val. ► Molecular modeling confirmed a better access of the amino acid moiety. ► New mixed selectors should be considered some of the most versatile for chiral GC.