Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7614827 | Journal of Chromatography B | 2018 | 24 Pages |
Abstract
In this study, a method for simultaneous determination of 40 antibiotics from 6 different drug families (7 quinolones, 5 penicillins, 8 macrolides, 2 lincosamides, 4 tetracyclines, and 13 sulfonamides) and 3 amantadines in animal-derived feedstuffs (ADF) through ultraperformance liquid chromatography-tandem mass spectrometry using a new lipid-removing solid-phase extraction column PRiME HLB was developed. The sample was extracted with 20â¯mL extraction solution and then purified and concentrated with PRiME HLB. The eluent was evaporated to dryness under nitrogen and analyzed with LC-MS/MS using acetonitrile and 0.1% formic acid as the mobile phase via gradient elution. Under the optimum conditions, recoveries were 65.8% to 104.4% with a relative standard deviation of <15% at spiked levels of 5.0â¯Î¼g/kg to 100â¯Î¼g/kg. The limits of detection ranged from 0.5â¯Î¼g/kg to 5â¯Î¼g/kg. 15 of the 43 drugs were found in ADF. Sulfonamides, fluoroquinolones, macrolides, tetracyclines, and amantadines were detected, with the highest levels reaching 325â¯Î¼g/kg.
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Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Suo Decheng, Wang Peilong, Li Yang, Wang Ruiguo, Wei Shulin, Xiao Zhiming, Zhang Su,