Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7614864 | Journal of Chromatography B | 2018 | 11 Pages |
Abstract
Tetanus is still a major cause of human deaths in several developing countries. In particular, the neonatal form remains a significant public health problem. According to the World Health Organization, administration of tetanus toxoid is recommended for neonatal tetanus patients. Furthermore, tetanus antitoxin or anti-tetanus immunoglobulin (Ig) are used for mild case or intensive care. This paper discusses a novel purification technique for improving equine anti-tetanus Ig production. First, equine plasma dealt with two steps salting out with ammonium sulfate; second, ultrafiltration concentration liquid purified by one successive protein G based affinity chromatography steps; finally, the purified F(abâ²)2 fragments was characterized using biochemical and proteomic methods and shown to be pure and homogeneous. Compared with the original technique product, specific activity increased by 80% (about 90,000â¯IU/g) and recovery of F(abâ²)2 is approximately equal 75%. Furthermore, Proteomic profiling of total technique process is demonstrated by nano-HPLC-MS and bioinformatics analysis. New technique to produce equine anti-tetanus immunoglobulin F(abâ²)2 fragments from crude plasma in high quality and yield. And it also could be used for industrial amplification.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Xue-Jun Zhang, Hai-Ling Li, Da-Yi Deng, Chong Ji, Xiao-Dong Yao, Jia-Xin Liu,