Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7614980 | Journal of Chromatography B | 2018 | 9 Pages |
Abstract
Biomedical research in areas such as metabolic disorders, neuromodulatory, and immunomodulatory conditions involves lipid metabolism and demands a reliable and inexpensive method for quantification of short chain fatty acids (SCFAs). We report a GC-MS method for analysis of all straight-chain and branched-chain SCFAs using pentafluorobenzyl bromide (PFBBr) as derivatization reagent. We optimized the derivatization and GC-MS conditions using a mixture containing all eight SCFA standards, i.e., five straight-chain and three branched-chain SCFAs. The optimal derivatization conditions were derivatization time 90â¯min, temperature 60â¯Â°C, pHâ¯7, and (CH3)2CO:H2O ratio 2:1 (v:v). Comparing the performance of different GC column configurations, a 30â¯m DB-225ms hyphenated with a 30â¯m DB-5ms column in tandem showed the best separation of SCFAs. Using the optimized experiment conditions, we simultaneously detected all SCFAs with much improved detection limit, 0.244-0.977â¯Î¼M. We further applied the developed method to measure the SCFAs in mouse feces and all SCFAs were successfully quantified. The recovery rates of the eight SCFAs ranged from 55.7% to 97.9%.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Liqing He, Md Aminul Islam Prodhan, Fang Yuan, Xinmin Yin, Pawel K. Lorkiewicz, Xiaoli Wei, Wenke Feng, Craig McClain, Xiang Zhang,