Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7615005 | Journal of Chromatography B | 2018 | 7 Pages |
Abstract
In this paper, the use of disposable pipette extraction (DPX) for the determination of pesticides in human urine in possible cases of poisoning is proposed for the first time. The pesticides studied were oxamyl, propoxur, carbofuran, 3âhydroxycarbofuran, carbaryl, methiocarb, terbufos, parathion methyl, malathion, chlorpyrifos and endosulfan. The pipette tip used for the extraction of these compounds was commercially acquired. It has a capacity of 5â¯mL and contains 20â¯mg of sorbent material (styrene-divinylbenzene). The optimization of the main parameters that can influence the extraction efficiency of this sample preparation technique was performed with univariate and multivariate approaches. The analytes were separated and identified by gas chromatography coupled to mass spectrometry (GC-MS). The optimal extraction conditions were 5 extraction cycles of 30â¯s and 5 desorption cycles of 15â¯s with 250â¯Î¼L of ethyl acetate. Elution of the extract was performed in a vial containing 100â¯mg of anhydrous sodium sulfate. The method developed was validated, providing correlation coefficients higher than 0.9955 for all analytes, limits of detection (LOD) of 0.76 to 1.52â¯Î¼gâ¯Lâ1, limits of quantification (LOQ) of 2.5 to 5.0â¯Î¼gâ¯Lâ1, relative recoveries of 63 to 118%, intra-day precision of 0.7 to 15.3% and inter-day precision of 4.9 to 13.1%. An effective and rapid method for the analysis of human urine for the identification of possible cases of poisoning by pesticides was successful developed.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Anderson Luiz Oenning, Josias Merib, Eduardo Carasek,