Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7615082 | Journal of Chromatography B | 2018 | 46 Pages |
Abstract
Sensitive LC-MS/MS methods were developed to measure lidocaine and its metabolite 2,6-dimethylaniline (2,6-DMA) with application to transdermal studies. The methods for lidocaine in minipig plasma, tissue biopsies, and dermal tapes utilized mixed mode/SCX solid phase extraction, with lower quantitation limits of 25â¯pg/mL in plasma, 15â¯ng/g tissue, and 5â¯ng/tape. 2,6-DMA was measured in plasma and skin tissue homogenates by ultrafiltration and (for tissue) by further derivatization with 4-methoxybenzoyl chloride to form the corresponding benzamide derivative, which extended the lower limit of quantitation to 200â¯pg/mL. The methods allowed local measurement of lidocaine in stratum corneum, punch biopsies, and plasma and of 2,6-DMA in plasma and biopsies obtained from minipigs dosed with experimental transdermal formulations. Quantitation limits were approximately 7-fold lower than previously reported for lidocaine and 3-fold lower for 2,6-DMA.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Qian Li, Tobias Magers, Brad King, Brian J. Engel, Ray Bakhtiar, Charisse Green, Ronald Shoup,