Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7615086 | Journal of Chromatography B | 2018 | 5 Pages |
Abstract
A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quantification of gefitinib in dried blood spots (DBSs). Gefitinib was extracted with methanol from DBS of 3â¯mm in diameter and detected using a triple quadrupole mass spectrometer. The method was validated by evaluating its precision, accuracy, selectivity, carryover, matrix effect, recovery, and stability. For clinical validation, paired finger-prick DBS and plasma concentrations were compared for 10 patients with non-small cell lung cancer (NSCLC) taking gefitinib. The calibration linear range was 37.5-2400â¯ng/mL (coefficient of determination [R2]â¯=â¯0.99), encompassing the therapeutic concentrations of gefitinib. The accuracy and precision were within 15% of the quality control (QC) concentrations of 80, 200, and 2000â¯ng/mL. The lower limit of quantification was determined to be 40â¯ng/mL. Gefitinib was stable in DBSs for up to 5â¯months at room temperature and â20â¯Â°C, and at 40â¯Â°C for 24â¯h. A good correlation was observed between the gefitinib levels measured by the DBS method and plasma concentrations (R2â¯=â¯0.99). This method provides a simple, fast, and accurate approach to the quantitative analysis of gefitinib in finger-prick DBSs. The method would be useful for minimally invasive evaluation of the clinical gefitinib blood concentration.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Kei Irie, Saori Shobu, Seika Hiratsuji, Yuta Yamasaki, Shigeki Nanjo, Chiyuki Kokan, Akito Hata, Reiko Kaji, Katsuhiro Masago, Shiro Fujita, Yutaka Okada, Nobuyuki Katakami, Shoji Fukushima,