Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7615151 | Journal of Chromatography B | 2018 | 33 Pages |
Abstract
Levonorgestrel (LNG) is a synthetic progestin that is available in oral contraceptive tablets, a subdermal implant, and an intrauterine system for contraception. LNG pharmacokinetics are a pivotal determinant of contraceptive efficacy and essential in assessing drug-drug interactions influencing LNG exposure following different routes of LNG administration. A highly sensitive LC-MS/MS method was developed and validated to quantify levonorgestrel in human plasma. Liquid-liquid extraction was utilized with a sample volume of 500â¯Î¼L to extract levonorgestrel from plasma. Chromatographic separation of LNG was achieved with a Fortis⢠C18 (3â¯Î¼m: 100â¯mmâ¯Ãâ¯2.1â¯mm) reverse phase analytical column. The mobile phases consisted of de-ionized water plus 0.1% NH4OH (100:0.1%, v/v) (A), and methanol plus 0.1% NH4OH (100:0.1%, v/v) (B) delivered as a gradient at a flow rate of 400â¯Î¼L/min. Detection of LNG and internal standard (D-(â)-norgestrel-d7) was achieved using positive polarity mode monitoring at 313.2-245.2â¯amu and 320.1-251.2â¯amu, respectively. The assay was linear over the calibration range of 49.6 to 1500â¯pg/mL. This method was used to quantify plasma LNG released by subdermal implant in support of a drug interaction study among women with HIV receiving efavirenz- or nevirapine-based antiretroviral therapy.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Lauren R. Cirrincione, Sujan Dilly Penchala, Kimberly K. Scarsi, Anthony T. Podany, Lee C. Winchester, David J. Back, Saye H. Khoo, Courtney V. Fletcher, Marco Siccardi, Laura J. Else,