Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7615161 | Journal of Chromatography B | 2018 | 9 Pages |
Abstract
Previous work from our laboratories utilized a novel skin taping method and mass spectrometry-based proteomics to discover clinical biomarkers of skin conditions; these included atopic dermatitis, Staphylococcus aureus colonization, and eczema herpeticum. While suitable for discovery purposes, semi-quantitative proteomics is generally time-consuming and expensive. Furthermore, depending on the method used, discovery-based proteomics can result in high variation and inadequate sensitivity to detect low abundant peptides. Therefore, we strove to develop a rapid, sensitive, and reproducible method to quantitate disease-related proteins from skin tapings. We utilized isotopically-labeled peptides and tandem mass spectrometry to obtain absolute quantitation values on 14 peptides from 7 proteins; these proteins had shown previous importance in skin disease. The method demonstrated good reproducibility, dynamic range, and linearity (R2â¯>â¯0.993) when nâ¯=â¯3 standards were analyzed across 0.05-2.5â¯pmol. The method was used to determine if differences exist between skin proteins in a small group of atopic versus non-atopic individuals (nâ¯=â¯12). While only minimal differences were found, peptides were detected in all samples and exhibited good correlation between peptides for 5 of the 7 proteins (R2â¯=â¯0.71-0.98). This method can be applied to larger cohorts to further establish the relationships of these proteins to skin disease.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Nichole Reisdorph, Michael Armstrong, Roger Powell, Kevin Quinn, Kevin Legg, Donald Leung, Rick Reisdorph,