Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7615278 | Journal of Chromatography B | 2018 | 9 Pages |
Abstract
An immunoaffinity clean-up material based on a monoclonal antibody (mAb) has been prepared for concentrating and purifying bisphenol A (BPA) in 14 kinds of foodstuffs at trace level. Haptens and immunogen of bisphenol A have been synthesized and comprehensively characterized. An mAb towards BPA was prepared and cross-reactivities with 14 BPA analogues were below 5%. The prepared antibody was coupled to N-hydroxysuccinimide-activated Sepharose 4B to manufacture an immunoaffinity column (IAC), which was applied to purify BPA in 14 kinds of foodstuffs. The analyte was then detected by means of ultra-high-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Under the optimized conditions, compared with two traditional SPE clean-up methods, the IAC showed better selectivity (matrix effect <16.8%) and higher sensitivity. The limits of detection for BPA in 14 kinds of foodstuffs ranged from 0.001â¯Î¼gâ¯Lâ1 to 0.01â¯Î¼gâ¯kgâ1, and the limits of quantification were in the range from 0.003â¯Î¼g Lâ1 to 0.04â¯Î¼gâ¯kgâ1. The recoveries of BPA from spiked samples ranged from 82.0% to 104.9%, with RSDs below 13.8%. Besides, the IAC exhibited good reusability, with 40% column capacity remaining and no significant loss of recovery after 25 application cycles in real sample detection. These results demonstrated that the developed IAC-UPLC-MS/MS approach has wide applicability for purifying and detecting BPA in various foodstuffs.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Kai Yao, Kai Wen, Wenchong Shan, Sanlei Xie, Tao Peng, Jianyi Wang, Haiyang Jiang, Bing Shao,