Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7615364 | Journal of Chromatography B | 2018 | 7 Pages |
Abstract
A simple, sensitive and selective LC-MS/MS method for quantitative analysis of human insulin was developed and validated in dog plasma. Insulin glargine was used as the internal standard. After a simple step of solid-phase extraction, the chromatographic separation of human insulin was achieved by using InertSustain Bio C18 column with a mobile phase of acetonitrile containing 1% formic acid (A)-water containing 1% formic acid (B). The detection was performed by positive ion electrospray ionization in multiple-reaction monitoring (MRM) mode. Good linearity was observed in the concentration range of 1-1000â¯Î¼IU/mL (r2â¯>â¯0.99), and the lower limit of quantification was 1â¯Î¼IU/mL (equal to 38.46â¯pg/mL). The intra- and inter-day precision (expressed as relative standard deviation, RSD) of human insulin were â¤12.1% and â¤13.0%, respectively, and the accuracy (expressed as relative error, RE) was in the range of â7.23-11.9%. The recovery and matrix effect were both within acceptable limits. This method was successfully applied for the pharmacokinetic study of human insulin in dogs after subcutaneous administration.
Related Topics
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Authors
Shiqi Dong, Yong Zeng, Guangli Wei, Duanyun Si, Changxiao Liu,