Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7615389 | Journal of Chromatography B | 2018 | 9 Pages |
Abstract
A new validated bio-analytical LC-MS/MS method was developed for the simultaneous extraction and determination of four proton pump inhibitors: esomeprazole, lansoprazole, pantoprazole and rabeprazole in human plasma using escitalopram as an internal standard. The proteins in plasma samples were precipitated using acetonitrile for the extraction of analytes which is a simple economic method. The separation was accomplished using a mobile phase composed of 10â¯mM ammonium formate: acetonitrile: methanol (20:40:40% v/v) at a flow rate of 0.8â¯mL/min in isocratic mode on a reversed phase C18 INERTSIL ODS-3 (5â¯Î¼m, 150â¯Ãâ¯4.6â¯mm) and column temperature of 40â¯Â°C. Positive mode electrospray ionization source was used prior to multiple reaction monitoring (MRM) detection using parent and daughter ions: m/z 346.2â¯ââ¯198.1 for esomeprazole, m/z 370.1â¯ââ¯252 for lansoprazole, m/z 384.2â¯ââ¯200.2 for pantoprazole, m/z 360.1â¯ââ¯242.1 for rabeprazole and m/z 325.2â¯ââ¯109 for escitalopram. The calibration curves were constructed, and the method was linear in the range of 20-5000â¯ng/mL applying weighted (1/X2) linear regression coefficient for all drugs. The method was fully validated following US-FDA and EMA guidelines.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ehab F. Elkady, Marwa A. Fouad, Bushra M. Jaadan,