| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7615713 | Journal of Chromatography B | 2018 | 26 Pages |
Abstract
Piperine was the metabolically most stable compound. Aliphatic hydroxylation, and N- and O-dealkylation were the major routes of oxidative metabolism. Piperine was exclusively metabolized by CYP1A2, whereas CYP2C9 contributed significantly in the oxidative metabolism of all analogs. Extensive binding to blood constituents was observed for all compounds.
Keywords
MRMTRPV1UHPLC-Q-TOF-MSCYP450ESIppbUHPLCQ-TOFDMSOinternal standardpotassium phosphate bufferCNSrapid equilibrium dialysisDimethyl sulfoxidequadrupole time-of-flightBBBblood brain barrierREDCytochrome P450central nervous systemMetabolite identificationMass spectrometryIn vitro metabolismmultiple reaction monitoringarbitrary unitstransient receptor potential vanilloid type 1high performance liquid chromatographyUltra-high performance liquid chromatographyHPLChigh resolutionGABAAelectrospray ionization
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Volha Zabela, Timm Hettich, Götz Schlotterbeck, Laurin Wimmer, Marko D. Mihovilovic, Fabrice Guillet, Belkacem Bouaita, Bénédicte Shevchenko, Matthias Hamburger, Mouhssin Oufir,