| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7615728 | Journal of Chromatography B | 2018 | 11 Pages |
Abstract
Here, a simple and cost effective N-glycosidase assay is presented, which is based on the direct determination of the released adenine by thin-layer chromatography (TLC) and TLC-densitometry. An adenine based single stranded oligonucleotide is used as substrate. Following TLC development the released adenine is quantified on silica glass plates by UV absorbance at 260Â nm.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Alexander Weng,