Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7615813 | Journal of Chromatography B | 2017 | 21 Pages |
Abstract
Astragali Radix, the root of Astragalus membranaceus Bunge, is one of the most frequently used crude drugs in Asian medicine. We developed a quantification method for 6 components (calycosin, formononetin, astragaloside I-IV) of Astragali Radix and Hwanggi-gyeji-omul-tang (HGOT) using reverse-phase high-performance liquid chromatography coupled with integrated pulsed amperometric detection (RP-HPLC-IPAD). The plants were extracted in 80% ethanol for 2Â h. All target components were detected with good sensitivity using sodium hydroxide (as a post-column eluent). The limit of detection (S/NÂ =Â 3) and limit of quantification (S/NÂ =Â 10) of the target components ranged from 0.10-1.00Â ng and from 0.30-3.00Â ng, respectively. The coefficients of linear regression ranged from 0.9993-1.0000, all interday and intraday precision values were <3.64%, and the average recovery ranged from 99.00-102.97% for Astragali Radix and 97.73-102.57% for HGOT. This method exhibited good selectivity, sensitivity, and reproducibility and can be used directly without any pretreatment steps. Our method will therefore be useful as a quality control measure for Astragali Radix.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Seung-Min Lee, Ji-Seon Jeong, Ha-Jeong Kwon, Seon-Pyo Hong,