Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7615833 | Journal of Chromatography B | 2017 | 11 Pages |
Abstract
The unintentional poisoning of off-target animals by bromadiolone, a second generation anticoagulant rodenticide, is an undesirable outcome requiring sensitive analytical methods. In this study, a rapid and sensitive method for the determination of bromadiolone in liver and blood plasma by means of gas chromatography coupled with tandem mass spectrometry without need for derivatization was developed. The method is based on the in-injector pyrolysis of bromadiolone and subsequent gas chromatography coupled with ion trap tandem mass spectrometry with electron ionization. Sample preparation includes extraction with methanol, evaporation under nitrogen stream, and dissolution in toluene. The pyrolysis of bromadiolone was carried out in an injector at 390 °C. Chromatographic separation of the pyrolytical fragment of bromadiolone was achieved using a VF-5 ms column with helium as the mobile phase. Tandem in-time mass spectrometry of the separated pyrolytical fragment of bromadiolone was carried out using an ion trap mass spectrometer after electron ionization. Recovery ranged from 94 to 98%. The method showed good linearity up to 1000 μg kgâ1 for liver and 1000 μg Lâ1 for plasma. The limit of detection was 0.38 μg kgâ1 for liver and 0.26 μg Lâ1 for plasma. The developed method was used successfully in several animal poisoning cases.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Veronika Doubková, Petr MarÅ¡álek, VladimÃr VeÄerek,