Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7615957 | Journal of Chromatography B | 2016 | 26 Pages |
Abstract
Multiple myeloma (MM), a malignant neoplastic serum-cell disorder, has been a serious threat to human health. The determination of 6 commonly used drug concentrations, including thalidomide, lenalidomide, cyclophosphamide, bortezomib, dexamethasone and adriamycin, in MM patients was of great clinical interest. Herein, we reported a method for the rapid and simultaneous measurement of the above therapeutics by liquid chromatography-tandem mass spectroscopy (LC-MS/MS) method with solid phase extraction. Analysis was performed on a Waters XBridge® BEH C18 column (2.5 μm, 2.1 mm Ã 50 mm), with formic acid aqueous solution and acetonitrile as the mobile phase at flow rate 0.3 mL/min. All analytes showed good correlation coefficients (r > 0.996), and LLOQ of thalidomide, lenalidomide, cyclophosphamide, bortezomib, dexamethasone and adriamycin were 4, 2, 2, 2, 2 and 2 ng/mL, respectively. The inter- and intra-day precisions and stability were expressed as variation coefficients within 15% and relative error less than 15%. Dilution effect, carryover and incurred sample reanalysis were investigated according to the 2015 edition Chinese Pharmacopoeia guidelines, as US FDA (2013, revision 1) required. The LC-MS/MS based assay described in this article may improve future clinical studies evaluating common therapeutics for MM treatment.
Keywords
ACNFLCLNDDXMTHDMELCTXADMPDNPPTLLEBTZLLOQLiquid chromatography-tandem mass spectroscopyUS FDALC–MS/MSAdriamycinU.S. Food and Drug AdministrationLiquid-liquid extractionAcetonitrileFormic acidSurveillance Epidemiology and End ResultsProtein precipitationBortezomibThalidomidelower limit of quantificationrelative errorAnti-cancer drugDexamethasoneHuman serumSEERCyclophosphamideFluconazoleLenalidomidemelphalanMultiple myelomaVindolinePrednisoneHigh performance liquid chromatography-tandem mass spectrometryquality control
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Chang Shu, Tianmei Zeng, Shouhong Gao, Tianyi Xia, Lifeng Huang, Feng Zhang, Wansheng Chen,