Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7616103 | Journal of Chromatography B | 2016 | 5 Pages |
Abstract
A rapid, simple and sensitive method was developed and validated using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for determination of albendazole sulfoxide (ABZOX) in human plasma. The plasma samples were extracted by protein precipitation using albendazole sulfoxide-d3 as internal standard (IS). The chromatographic separation was performed on Waters Xbridge C18Column (100 Ã 4.6 mm, 3.5 μm) with a mobile phase consisting of ammonia solution, water and methanol at a flow rate of 0.70 mL/min. ABZOX was detected and identified by mass spectrometry with electrospray ionization (ESI) in positive ion and multiple-reaction monitoring (MRM) mode. The method was linear in the range of 3-1500 ng/mL for ABZOX. This method was successfully applied to the bioequivalence study in human plasma samples.
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Authors
Nihal Saraner, Güler YaÄmur Ãzkan, Berrak Güney, Erkin Alkan, Nihan Burul-Bozkurt, Onursal SaÄlam, Ezgi FikirdeÅici, Mevlüt Yıldırım,