Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7616467 | Journal of Chromatography B | 2016 | 10 Pages |
Abstract
Neurotransmitters (NTs) and their metabolites are known to play an essential role in maintaining various physiological functions in nervous system. However, there are many difficulties in the detection of NTs together with their metabolites in biological samples. A new method for NTs and their metabolites detection by high performance liquid chromatography coupled with Q Exactive hybrid quadruple-orbitrap high-resolution accurate mass spectrometry (HPLC-HRMS) was established in this paper. This method was a great development of the applying of Q Exactive MS in the quantitative analysis. This method enabled a rapid quantification of ten compounds within 18Â min. Good linearity was obtained with a correlation coefficient above 0.99. The concentration range of the limit of detection (LOD) and the limit of quantitation (LOQ) level were 0.0008-0.05Â nmol/mL and 0.002-25.0Â nmol/mL respectively. Precisions (relative standard deviation, RSD) of this method were at 0.36-12.70%. Recovery ranges were between 81.83% and 118.04%. Concentrations of these compounds in mouse hypothalamus were detected by Q Exactive LC-MS technology with this method.
Keywords
5-HTDNS-Cll-AA3-methoxy-4-hydroxyphenylglycolMHPG5-hydroxytryptophanNTS5-HIAADOPACQ-TOF5-hydroxytryptamineMRM5-HTPLOQSRM3,4-dihydroxyphenylacetic acid5-hydroxyindole-3-acetic acidl-TyrosineLC–MS/MSinternal standardγ-aminobutyric acidNeurotransmittersEpinephrineQuantitative detectionLOD یا Limit of detectionDansyl chlorideDopaminequadrupole time-of-flightCoefficient of VariationMass spectrometrylimit of quantificationlimit of detectionMouseselected reaction monitoringmultiple reaction monitoringnorepinephrinehomovanillic acidHypothalamusHVACaffeic acidliquid chromatography coupled with tandem mass spectrometryHPLChigh-performance liquid chromatographyquality controlGABA
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Zong-Lin Yang, Hui Li, Bing Wang, Shu-Ying Liu,