Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7616905 | Journal of Chromatography B | 2015 | 7 Pages |
Abstract
Ipragliflozin is a highly potent and selective sodium-dependent glucose co-transporter-2 (SGLT2) inhibitor, a novel class of hypoglycemic agents. The aim of the present study was to establish a new highly sensitive and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantitative analysis of ipragliflozin in rat plasma and apply this method to a pharmacokinetic study in rats. Empagliflozin was used as an internal standard (I.S.) and liquid-liquid extraction was conducted using tert-butyl methyl ether. Chromatographic separation was accomplished on a Quicksorb ODS (2.1 mm i.d. Ã 150 mm, 5 μm in size) with acetonitrile/0.1% formic acid (90:10, v/v) at a flow rate of 0.2 mL/min. An API 3200 triple quadrupole mass spectrometer operating in the positive electrospray ionization mode with multiple reaction monitoring was used to detect ipragliflozin and I.S. transitions: m/z 422.0 [M + NH4]+ â 151.0 for ipragliflozin and m/z 451.2 [M + H]+ â 71.0 for I.S. Inter- and intra-day accuracies and precisions were within ±15%. This validated method was successfully applied to a pharmacokinetic study of ipragliflozin in rats. This assay method may contribute to assessment of novel SGLT2 inhibitors using the rat as an animal model.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Shinji Kobuchi, Yukako Ito, Kyoka Yano, Toshiyuki Sakaeda,