Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7617016 | Journal of Chromatography B | 2015 | 34 Pages |
Abstract
A rapid and sensitive high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to simultaneous quantify artesunate and its metabolite in sheep plasma. The plasma samples were prepared by liquid-liquid extraction. Chromatographic separation was achieved on a C18 column (250 Ã 4.6 mm, 5 μm) using methanol: water (60:40, v/v) (the water included 1 mM ammonium acetate, 0.1% formic acid, and 0.02% acetic acid) as the mobile phase. Mass detection was carried out using positive electrospray ionization in multiple reaction monitoring mode. The calibration curve was linear from 1 ng/mL to 400 ng/mL (r2 = 0.9992 for artesunate, r2 = 0.9993 for its metabolite). The intra- and inter-day accuracy and precision were within the acceptable limits of ±10% at all concentrations for both artesunate and its metabolite. The recoveries ranged from 92% to 98% at the three concentrations for both. In summary, the LC-MS/MS metho described herein was fully successfully applied to pharmacokinetic studies of artesunate nanoemulsion after intramuscular delivery to sheep.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Bing Li, Jie Zhang, Xu-Zheng Zhou, Jian-Yong Li, Ya-Jun Yang, Xiao-Juan Wei, Jian-Rong Niu, Xi-Wang Liu, Jin-Shan Li, Ji-Yu Zhang,