Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7617273 | Journal of Chromatography B | 2015 | 25 Pages |
Abstract
Picrasma quassioides (D. Don) Benn. is used in traditional Chinese medicine for the treatment of inflammation. Characteristic components of the medicinal extract are canthinone alkaloids. In this study, a sensitive and rapid liquid chromatography with tandem mass spectrometry method has been developed for simultaneous quantification of two major canthinone alkaloids, 5-hydroxy-4-methoxycanthin-6-one and 4,5-dimethoxycanthin-6-one, in rat plasma after oral administration of P. quassioides extract (200Â mg/kg). The chromatographic separation was performed on a C18 column using acetonitrile-aqueous 0.1% formic acid (90:10, v/v) as the mobile phase. Plasma samples were prepared for analysis using a simple liquid-liquid extraction with ethyl acetate. Analytes were detected using tandem mass spectrometry in positive multiple reaction monitoring mode. Method validation revealed excellent linearity over the range 1.25-900Â ng/mL for 5-hydroxy-4-methoxycanthin-6-one and 0.5-800Â ng/mL for 4,5-dimethoxycanthin-6-one with satisfactory intra- and inter-day precision, accuracy and recovery. Samples were stable under the conditions tested. The pharmacokinetic profiles of the analytes in rats showed that both canthinones were rapidly absorbed and that 4,5-dimethoxycanthin-6-one was eliminated faster than 5-hydroxy-4-methoxycanthin-6-one.
Keywords
LLOQFDACIDSRMULOQRSDMRMHPLC–MS/MSCollision-induced dissociationinternal standardMethod validationrelative standard deviationupper limit of quantificationlower limit of quantificationrelative errorFood and Drug AdministrationPharmacokineticsselected reaction monitoringmultiple reaction monitoringquality control
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yuanyuan Shi, Chunyan Hong, Jian Xu, Xiaoling Yang, Ning Xie, Feng Feng, Wenyuan Liu,