Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7617463 | Journal of Chromatography B | 2014 | 6 Pages |
Abstract
A fast, sensitive and specific hydrophilic interaction chromatography combined with tandem mass spectrometry (HILIC-MS/MS) method was developed for the determination of tobramycin in human plasma. With sisomycin as internal standard, the analysis was carried out on a hilic column (150 mm Ã 2.1 mm, 3.5 μm) using a mobile phase consisting of acetonitrile: 5 mM ammonium acetate and 0.1% formic acid (60:40, v/v). The detection was performed by tandem spectrometry via electrospray ionization (ESI). Linear calibration curves were obtained in the concentration range of 10.51-1051 ng/mL for tobramycin, with a lower limit of quantification of 10.51 ng/mL. The intra- and inter-day precision (RSD) values were below 15% and accuracy (RE) was 1.3-5.7% at all QC levels. The method was applicable to the clinical study of the pharmacokinetics of tobramycin in healthy volunteers.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Lingyun Chen, Huashan Chen, Mei Shen,