Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7617517 | Journal of Chromatography B | 2014 | 31 Pages |
Abstract
A liquid chromatography-tandem mass spectrometry assay requiring a 100 μL aliquot of human plasma for simultaneous determination of rilpivirine, a second generation non-nucleoside reverse transcriptase inhibitors of HIV and dolutegravir, a novel integrase stand transfer inhibitors of HIV concentrations has been developed. Sample pre-treatment is limited to protein precipitation with a mixture of methanol and zinc sulfate. After centrifugation the supernatant is injected in the chromatographic system, which consists of on-line solid phase extraction followed by separation on a phenyl-hexyl column. This 2.5 min method, with its simple sample preparation provides sensitive (the limit of quantitation is 25 ng/mL for each compound), accurate and precise (the intra-day and inter-day imprecision and inaccuracy are lower than 15%) quantification of the plasma concentration of these drugs and can be used for therapeutic drug monitoring in patients infected with HIV.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
M. Grégoire, G. Deslandes, C. Renaud, R. Bouquié, C. Allavena, F. Raffi, P. Jolliet, E. Dailly,