Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7617916 | Journal of Chromatography B | 2014 | 8 Pages |
Abstract
Ptaquiloside (PTA) is a toxin from bracken fern (Pteridium sp.) with genotoxic effects. Hydrolysis of PTA leads to the non-toxic and aromatised indanone, pterosin B (PTB). Here we present a sensitive, fast, simple and direct method, using SPE cartridges to clean and pre-concentrate PTA and PTB in plasma, urine and milk followed by LC-MS quantification. The average recovery of PTA in plasma, urine, and milk was 71, 88 and 77%, respectively, whereas recovery of PTB was 75, 82 and 63%. The method LOQ for PTA and PTB in plasma was 1.2 and 3.7 ng mLâ1, 52 and 33 ng mLâ1 for undiluted urine and 5.8 and 5.3 ng mLâ1 for milk. The method is repeatable within and between days, with RSD values lower than 15% (PTA) and 20% (PTB). When PTA and PTB spiked samples were stored at â18 °C for 14 days both compounds remained stable. In contrast, the PTA concentration was reduced by 15% when PTA spiked plasma was left for 5 h at room temperature before SPE clean-up, whereas PTB remained stable. The method is the first to allow simultaneous quantification of PTA and PTB in biological fluids in a relevant concentration range. After intravenous administration of 0.092 mg PTA per kg bw in a heifer, the plasma concentration was more than 300 ng mLâ1 PTA and declined to 9.8 ng mLâ1 after 6 h, PTB was determined after 10 min at 50 ng mLâ1
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Paulo César Reis Aranha, Hans Christian Bruun Hansen, Lars Holm Rasmussen, Bjarne W. Strobel, Christian Friis,