Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7618322 | Journal of Chromatography B | 2013 | 21 Pages |
Abstract
A simple and rapid method for the simultaneous determination of serum nicotine and cotinine using high-performance liquid chromatography (HPLC)-fluorometric detection with a postcolumn ultraviolet-photoirradiation system was developed. Analytes were extracted from alkalinized human serum via liquid-liquid extraction using chloroform. The organic phase was back-extracted with the acidified aqueous phase, and the analytes were directly injected into an ion-pair reversed-phase HPLC system. 6-Aminoquinoline was used as an internal standard. Nicotine, cotinine, and 6-aminoquinoline were separated within 14Â min. The extraction efficiency of nicotine and cotinine was greater than 91%. The linear range was 0.30-1000Â ng for nicotine and 0.06-1000Â ng for cotinine. In serum samples from smokers, the concentrations of nicotine and cotinine were 8-15Â ng/mL and 156-372Â ng/mL, respectively.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Makoto Yasuda, Tatsuhiro Ota, Atsushi Morikawa, Ken-ichi Mawatari, Tomoko Fukuuchi, Noriko Yamaoka, Kiyoko Kaneko, Kazuya Nakagomi,