Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
7618494 | Journal of Chromatography B | 2012 | 9 Pages |
Abstract
⺠Developed a simple SDS-assisted sample preparation method for shotgun analysis of membrane proteomes. ⺠Utilized the strong solubilization ability of SDS for the extraction and solubilization of membrane proteins. ⺠Optimized KDS precipitation for high efficiency of SDS-removal and peptide recovery. ⺠The developed method obviously improves the identification of membrane proteins particularly IMPs. ⺠The developed method is easy to operate at low cost.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yong Lin, Huajun Jiang, Yujun Yan, Bin Peng, Jinhua Chen, Haiyan Lin, Zhonghua Liu,