Comparison of ZIC-HILIC and graphitized carbon-based analytical approaches combined with exoglycosidase digestions for analysis of glycans from monoclonal antibodies

► Two LC approaches for analysis of therapeutic monoclonal antibodies are compared. ► ZIC-HILIC enables identification of major glycan species using fluorescence or ESI-MS detection. ► Porous graphitized carbon improves sensitivity, providing information on minor species. ► Fluorescence-based approaches offer simpler data analysis for routine glycan profiling. ► MS-based approaches provide detailed glycosylation analysis of complex glycoprotein samples.