| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7618620 | Journal of Chromatography B | 2012 | 8 Pages |
Abstract
⺠A sensitive nucleotide pyrophosphatase (NPP) assay was developed. ⺠p-Nitrophenyl 5â²-thymidine monophosphate was used as an artificial substrate. ⺠A CE-VIS method coupled with dynamically polybrene-coated capillaries was used. ⺠A combination of phosphate-HEPES buffer notably increased the method sensitivity. ⺠The obtained LOD and LOQ for p-nitrophenolate are in the nanomolar range.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Sang-Yong Lee, Sébastien A. Lévesque, Jean Sévigny, Christa E. Müller,