| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7618878 | Journal of Chromatography B | 2012 | 11 Pages |
Abstract
⺠Four extraction protocols were compared to optimize bile acid quantification by HPLC MS/MS. ⺠The best protocols were the Acetonitrile one for liquid (serum, urine, bile) and the NaOH one for stool samples. ⺠Allowed to detect 14 different species in serum, 11 in urine and 25 in stool. ⺠Applied to control and cholestatic patients and useful for quantification in routine analysis.
Keywords
HCAGlcAUDCAHDCACDCATCDCAGCAGDCATCASPETDCADCATLCALOQGUDCAGCDCAGC–MSHPLC–MS/MSLCAExtractionSolid phase extractionChenodeoxycholic acidDeoxycholic acidTaurocholic acidLithocholic acidHyodeoxycholic acidhyocholic acidCholic acidGlycodeoxycholic acidGlycochenodeoxycholic acidglycolithocholic acidglycocholic acidBile acidsUrsodeoxycholic acidRoutine analysisrelative errorCoefficient of Variationliquid chromatography–mass spectrometrylimit of quantificationProfilinggas chromatography coupled to mass spectrometry
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Lydie Humbert, Marie Anne Maubert, Claude Wolf, Henri Duboc, Myriam Mahé, Dominique Farabos, Philippe Seksik, Jean Maurice Mallet, Germain Trugnan, Joëlle Masliah, Dominique Rainteau,