| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7619112 | Journal of Chromatography B | 2012 | 8 Pages |
Abstract
⺠We purified a plant-produced HPV16 subunit vaccine candidate. ⺠High purity and good overall process yield was achieved for the product. ⺠The process was scaled-up from 50 g to 1 kg biomass scale. ⺠Product quality was confirmed by a diverse set of analysis methods.
Keywords
MALLSBiopharmaceuticalIMACMWCODTTDCsORFPAAPAGEMMCLICSECPOILOQESISDSHPV16PBSPolyacrylamideCervical intraepithelial neoplasiapolyacrylamide gel electrophoresisHICTransient expressionCinRoom temperaturedithiothreitolcircular dichroismcolumn volumesodium dodecyl sulfateDendritic cellsMass spectrometryPhosphate buffered salineopen reading framelysogeny brothLichenaselimit of quantificationPlant-produced vaccineMolecular Weight Cut OffHuman papillomavirusHPVPurificationmulti-angle laser light scatteringProtein of interestRecombinant proteinSize exclusion chromatographyhydrophobic interaction chromatographyIon exchange chromatographyimmobilized metal affinity chromatographyIECElectro spray ionization
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
J.F. Buyel, J.A. Bautista, R. Fischer, V.M. Yusibov,