| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 7619126 | Journal of Chromatography B | 2012 | 7 Pages |
Abstract
⺠A fluorescent-based assay for the quantification of thiol adducts is described. ⺠E. coli-derived recombinant proteins require refolding for activity. ⺠Cysteine and cystamine from the redox-couple can generate thiol-protein adducts. ⺠Released thiols are derivatized and separated by reversed-phase HPLC. ⺠Quantification of thiol adducts is accomplished by comparison to standard curves.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Brian D. Soriano, Lei-Ting T. Tam, Hsieng S. Lu, Violeta G. Valladares,