Rapid quantification of histamine in human psoriatic plaques using microdialysis and ultra high performance liquid chromatography with fluorescence detection

► Development of a rapid and ultra-sensitive liquid chromatographic method for the separation and detection of histamine. ► This represents one of the fastest reported separations of histamine using fluorescence detection. ► Intracutaneous histamine was measured 70 min after catheter implantation, a second fraction was collected 190 min after implantation and revealed similar levels with no difference in intracutaneous histamine between the control, peri-lesional and lesional skin regions. ► When combined with a novel rapid sensitive analytical method microdialysis may be used to explore the role of mast cells in psoriasis.