Quantification of 5-hydroxymethylfurfural in honey samples and acidic beverages using spectrophotometry coupled with ultrasonic-assisted cloud point extraction

- A simple method is proposed to quantify trace 5-HMF in honey and beverages.
- The method is based on the complexation of 5-HMF with p-NPH at pH 4.0.
- It is an interesting study with a significant impact on food safety.
- Detection limit, 1.96 μg L−1, in linear range of 6.5-275 μg L−1.
- The method also can be extended to 5-HMF determination in real time samples.